Nancy Denslow
Associate Professor, Department of Physiological Sciences
Department of Physiological Sciences
University of Florida
PO Box 100144, 1600 SW Archer Rd
Gainesville, FL 32610-0144
USA
Titles
- Associate Professor, Department of Physiological Sciences
Biography
Dr. Denslow's research interests include discovering biomarkers at both the mRNA and protein levels that relate to exposure of fish to environmental contaminants. In particular, she is interested in defining molecular mechanisms of action of endocrine disrupting compounds that adversely affect reproduction. Her research covers both sex hormone receptor mediated and independent mechanisms. Favorite model systems include largemouth bass, fathead minnow, sheepshead minnow and zebrafish. Common research tools include microarrays, real time PCR, proteomics, tissue culture based assays, transfections and in vivo determination of reproductive endpoints.
In addition, Dr. Denslow has initiated research to understand the effect of nanomaterials on fish health. For this research, zebrafish microarray analysis is used as a tool to suggest global pathways that may be affected by exposure. These experiments are integrated with others looking at gill function, histopathology, nanomaterial uptake and nanomaterial characterization.
Education
- MS, Biochemistry, Yale University
- PhD, Molecular Biology, University of Florida
Journal Articles
2018
Dang, V. D., K. J. Kroll, S. D. Supowit, R. U. Halden and N. D. Denslow. 2018. Activated carbon as a means of limiting bioaccumulation of organochlorine pesticides, triclosan, triclocarban, and fipronil from sediments rich in organic matter. Chemosphere 197(Apr):627-633. DOI: 10.1016/j.chemosphere.2018.01.062. (link )
2016
Dang, V. D., K. J. Kroll, S. D. Supowit, R. U. Halden and N. D. Denslow. 2016. Bioaccumulation of legacy and emerging organochlorine contaminants in Lumbriculus variegatus. Archives of Environmental Contamination and Toxicology DOI: 10.1007/s00244-016-0264-x. (link )
Dang, V. D., K. J. Kroll, S. D. Supowit, R. U. Halden and N. D. Denslow. 2016. Tissue distribution of organochlorine pesticides in largemouth bass (Micropterus salmoides) from laboratory exposure and a contaminated lake. Environmental Pollution 216(Sep):877-883. DOI: 10.1016/j.envpol.2016.06.061. (link )
Supowit, S. D., I. B. Roll, V. D. Dang, K. J. Kroll, N. D. Denslow and R. U. Halden. 2016. Active sampling device for determining pollutants in surface and pore water -- the in situ sampler for biphasic water monitoring. Scientific Reports 6:21886. DOI: 10.1038/srep21886. (link )
2015
Groh, K. J., R. N. Carvalho, J. K. Chipman, N. D. Denslow, M. Halder, C. A. Murphy, D. Roelofs, A. Rolaki, K. Schirmer and K. H. Watanabe. 2015. Development and application of the adverse outcome pathway framework for understanding and predicting chronic toxicity: I. Challenges and research needs in ecotoxicology. Chemosphere 120(Feb):764-777. DOI: 10.1016/j.chemosphere.2014.09.068. (link )
Groh, K. J., R. N. Carvalho, J. K. Chipman, N. D. Denslow, M. Halder, C. A. Murphy, D. Roelofs, A. Rolaki, K. Schirmer and K. H. Watanabe. 2015. Development and application of the adverse outcome pathway framework for understanding and predicting chronic toxicity: II. A focus on growth impairment in fish. Chemosphere 120(Feb):778-792. DOI: 10.1016/j.chemosphere.2014.10.006. (link )
Halden, R. U., E. M. Hartmann, N. D. Denslow, P. A. Haynes and J. LaBear. 2015. Recent advances in proteomics applied to elucidate the role of environmental impacts on human health and organismal function. Journal of Proteome Research 14(1):1-4. DOI: 10.1021/pr501224f. (link )
2012
Villeneuve, D. L., N. Garcia-Reyero, D. Martinovic-Weigelt, Z. Li, K. H. Watanabe, E. F. Orlando, C. A. LaLone, S. W. Edwards, L. D. Burgoon, N. D. Denslow, E. J. Perkins and G. T. Ankley. 2012. A graphical systems model and tissue-specific functional gene sets to aid transcriptomic analysis of chemical impacts on the female teleost reproductive axis. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 746(2):151-162. DOI: 10.1016/j.mrgentox.2011.12.016. (link )
2011
Johns, S. M., N. D. Denslow, M. D. Kane, K. H. Watanabe, E. F. Orlando and M. S. Sepulveda. 2011. Effects of estrogens and antiestrogens on gene expression of fathead minnow (Pimephales promelas) early life stages. Environmental Toxicology 26(2):195-206. DOI: 10.1002/tox.20545. (link )
Li, Z., K. J. Kroll, K. Jensen, D. L. Villeneuve, G. T. Ankley, J. V. Brian, M. S. Sepulveda, E. F. Orlando, J. M. Lazorchak, M. Kostich, B. Armstrong, N. D. Denslow and K. H. Watanabe. 2011. A computational model of the hypothalamic-pituitary-gonadal axis in female fathead minnows (Pimephales promelas) exposed to 17α-ethynylestradiol and 17β-trenbolone. BMC Systems Biology 5:Art. 63. DOI: 10.1186/1752-0509-5-63. (link )
2009
Ankley, G. T., D. C. Bencic, M. S. Breen, T. W. Collette, R. B. Conolly, N. D. Denslow, S. W. Edwards, S. Ekman, N. Garcia-Reyero, K. M. Jensen, J. M. Lazorchak, D. Martinovic-Weigelt, D. H. Miller, E. J. Perkins, E. F. Orlando, D. L. Villeneuve, R. Wang and K. H. Watanabe. 2009. Endocrine disrupting chemicals in fish: Developing exposure indicators and predictive models of effects based on mechanism of action. Aquatic Toxicology 92(3):168-178. DOI: 10.1016/j.aquatox.2009.01.013. (link )
Garcia-Reyero, N., K. J. Kroll, L. Liu, E. F. Orlando, K. H. Watanabe, M. S. Sepulveda, D. L. Villeneuve, E. J. Perkins, G. T. Ankley and N. D. Denslow. 2009. Gene expression responses in male fathead minnows exposed to binary mixtures of an estrogen and antiestrogen. BMC Genomics 10:Art. 308. DOI: 10.1186/1471-2164-10-308. (link )
Garcia-Reyero, N., D. L. Villeneuve, K. J. Kroll, L. Liu, E. F. Orlando, K. H. Watanabe, M. S. Sepulveda, G. T. Ankley and N. D. Denslow. 2009. Expression signatures for a model androgen and antiandrogen in the fathead minnow (em>Pimephales promelas ovary. Environmental Science & Technology 43(7):2614-2619. DOI: 10.1021/es8024484. (link )
Johns, S. M., M. D. Kane, N. D. Denslow, K. H. Watanabe, E. F. Orlando, D. L. Villeneuve, G. T. Ankley and M. S. Sepulveda. 2009. Characterization of ontogenetic changes in gene expression in the fathead minnow (Pimephales promelas). Environmental Toxicology and Chemistry 28(4):873-880. DOI: 10.1897/08-213R.1. (link )
Watanabe, K. H., Z. Li, K. J. Kroll, D. L. Villeneuve, N. Garcia-Reyero, E. F. Orlando, M. S. Sepulveda, T. W. Collette, D. R. Ekman, G. T. Ankley and N. D. Denslow. 2009. A computational model of the hypothalamic-pituitary-gonadal axis in male fathead minnows exposed to 17α-ethinylestradiol and 17β-estradiol. Toxicological Sciences 109(2):180-192. DOI: 10.1093/toxsci/kfp069. (link )